Astragaloside98%-physico-chemical Property

- Jan 11, 2018 -

Astragaloside is a high purity drug extracted from Astragalus, "Super Astragalus polysaccharide," said. Astragaloside can enhance the body's immune system, improve the body's resistance to disease.

【Appearance】: white crystalline powder

[Purity]: 98% or more, detection method HPLC.

【Plant origin】: Leguminosae Astragalus alexandrinus Boiss root, root of Astragalus Astragalus dissectus, Astragalus membranaceus (Fisch.) Bungede root, Astragalus sieversianus Pall., Astragalus spinosus Vahl Aboveground.

【Product Properties】: Astragalus extract for the brown powder.

[Determination]: According to high performance liquid chromatography ("Chinese Pharmacopoeia" 2010 edition an appendix Ⅵ D) determination.

Chromatographic conditions and system suitability test octadecylsilane bonded silica as a filler, acetonitrile - water (32:68) as the mobile phase; evaporative light scattering detector detection. The number of theoretical plates calculated Astragaloside peak should be not less than 4000.

Preparation of reference solution Take appropriate amount of astragaloside reference standard, accurately weighed, add methanol made per 1ml containing 0.5mg solution, that is, too.

Preparation of the test solution to take this product powder about 4g, accurately weighed, set Soxhlet extractor, add methanol 40ml, cold dip overnight, plus methanol amount, heated to reflux for 4 hours, the extract was recovered solvent and concentrated to Dry, the residue add water 10ml, slightly heated to dissolve, n-butanol saturated with water, shake extraction 4 times, each 40ml, combined n-butanol solution, with ammonia test solution 2 times, each 40ml, , N-butanol solution was evaporated to dryness, the residue 5ml of water to dissolve, let cool, through the D101 macroporous resin column (inner diameter of 37.5px, column height of 300px), eluted with water 50ml, 40% ethanol 30ml elution, discard the eluate, followed by 70% ethanol 80ml elution, the eluate was collected, evaporated to dryness, the residue dissolved in methanol, transferred to a 5ml volumetric flask, add methanol to the mark, shake, That is to say.

Determination of precision drawing reference solution 10μl, 20μl, for the test solution of 20μl, into the liquid chromatograph, determination, with an external standard two-point logarithm equation, that is, too.

This product is calculated on dry goods, with astragaloside (C41H68O14) shall not be less than 0.040%. [1]


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